Uricase antibody was used to detect purified uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed (lot 6732). Primary antibody was detected with Dylight 649 conjugated donkey anti-goat secondary antibody.