Detection of endogenous MET: Whole cell extracts of EGF stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MET antibody clone 14G9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
Detection of endogenous MET: Whole cell extracts of EGF stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MET antibody clone 14G9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2
MET (Activation Loop) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14G9]
AM20218PU-N
Overview
- SupplierOriGene
- Product NameMET (Activation Loop) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14G9]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID14G9
- Gene ID4233
- Target nameMET
- Target descriptionMET proto-oncogene, receptor tyrosine kinase
- Target synonymsAUTS9; c-Met; DFNB97; hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; proto-oncogene c-Met; RCCP2; scatter factor receptor; SF receptor; tyrosine-protein kinase Met
- HostMouse
- IsotypeIgG1
- Protein IDP08581
- Protein NameHepatocyte growth factor receptor
- Scientific DescriptionMET (Activation Loop) (incl. pos. control) mouse monoclonal antibody, clone 14G9, Purified
- ReactivityHuman
- UNSPSC12352203