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MID1 Antibody (aa575-603, Azide-free, HRP)

LS-C263154
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMID1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MID1 Antibody (aa575-603, Azide-free, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID4281
  • Target name
    MID1
  • Target description
    midline 1
  • Target synonyms
    BBBG1; E3 ubiquitin-protein ligase Midline-1; FXY; GBBB1; MIDIN; midline 1 RING finger protein; OGS1; Opitz/BBB syndrome; OS; OSX; putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; RNF59; TRIM18; tripartite motif protein TRIM18; tripartite motif-containing protein 18; XPRF; zinc finger on X and Y, mouse, homolog of; ZNFXY
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MID1 using anti-MID1 antibody (A01774-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK29 whole cell lysates Lane 2: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MID1 antigen affinity purified polyclonal antibody (Catalog # A01774-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MID1 at approximately 75KD. The expected band size for MID1 is at 75KD.
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