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ProductsBack/Milk Fat Globule EGF Factor 8 (MFGE8) Polyclonal Antibody

Milk Fat Globule EGF Factor 8 (MFGE8) Polyclonal Antibody

CAU26207
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMfge8
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Overview

  • Supplier
    Biomatik
  • Product Name
    Milk Fat Globule EGF Factor 8 (MFGE8) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID17304
  • Target name
    Mfge8
  • Target description
    milk fat globule EGF and factor V/VIII domain containing
  • Target synonyms
    AA408458; AI325141; EGF/factor VIII; lacta; lactadherin; MFG-E8; Mfgm; milk fat globule glycoprotein; milk fat globule-EGF factor 8 protein; MP47; P47; SED; SED1; sperm surface protein SP47
  • Host
    Rabbit
  • Protein IDP21956
  • Protein Name
    Lactadherin
  • Scientific Description
    The Milk Fat Globule EGF Factor 8 (MFGE8) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Milk Fat Globule 1/Mfge8 using anti-Milk Fat Globule 1/Mfge8 antibody (A02518-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/Mfge8 antigen affinity purified polyclonal antibody (Catalog # A02518-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/Mfge8 at approximately 65-70KD. The expected band size for Milk Fat Globule 1/Mfge8 is at 65-70KD.
Product group Antibodies
Boster Bio
Anti-Milk Fat Globule 1/Mfge8 Antibody Picoband(r)A02518-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetMfge8
  • SizePrice