Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLANA/MART1 Polyclonal Antibody (bs-0051R) at 1:400 overnight at 4°C, followed by SP Kit (Rabbit) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLANA/MART1 Polyclonal Antibody (bs-0051R) at 1:400 overnight at 4°C, followed by SP Kit (Rabbit) according to instructions and DAB staining.
MLANA/MART1 Polyclonal Antibody
BS-0051R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMLANA
Overview
- SupplierBioss Antibodies
- Product NameMLANA/MART1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2315
- Target nameMLANA
- Target descriptionmelan-A
- Target synonymsantigen LB39-AA; antigen SK29-AA; epididymis secretory sperm binding protein; MART1; MART-1; melanoma antigen recognized by T-cells 1; protein Melan-A
- HostRabbit
- IsotypeIgG
- Protein IDQ16655
- Protein NameMelanoma antigen recognized by T-cells 1
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203