Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLK3(Thr277 + Ser281) Antibody, Unconjugated (bs-3280R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
MLK3(Thr277 + Ser281) Antibody
BS-3280R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetMAP3K11
Overview
- SupplierBioss Antibodies
- Product NameMLK3(Thr277 + Ser281) Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID4296
- Target nameMAP3K11
- Target descriptionmitogen-activated protein kinase kinase kinase 11
- Target synonymsMEKK11; mitogen-activated protein kinase kinase kinase 11; mixed lineage kinase 3; MLK3; MLK-3; protein-tyrosine kinase PTK1; PTK1; SH3 domain-containing proline-rich kinase; SPRK; src-homology 3 domain-containing proline-rich kinase
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDQ16584
- Protein NameMitogen-activated protein kinase kinase kinase 11
- ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203