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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLK3(Thr277 + Ser281) Antibody, Unconjugated (bs-3280R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLK3(Thr277 + Ser281) Antibody, Unconjugated (bs-3280R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLK3(Thr277 + Ser281) Antibody, Unconjugated (bs-3280R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

MLK3(Thr277 + Ser281) Antibody

Research Use Only
BS-3280R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetMAP3K11
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MLK3(Thr277 + Ser281) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4296
  • Target name
    MAP3K11
  • Target description
    mitogen-activated protein kinase kinase kinase 11
  • Target synonyms
    MEKK11; mitogen-activated protein kinase kinase kinase 11; mixed lineage kinase 3; MLK3; MLK-3; protein-tyrosine kinase PTK1; PTK1; SH3 domain-containing proline-rich kinase; SPRK; src-homology 3 domain-containing proline-rich kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Modification Type
    Phosphorylated
  • Protein IDQ16584
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 11
  • Reactivity
    Bovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203