ABscript II cDNA First Strand Synthesis

Catalog number: MBS9141671_50reactions
Brand: MyBioSource
Packing: 50 reactions
Price: € 367.00
Expected delivery time: 21 days

Product specifications for - ABscript II cDNA First Strand Synthesis

Product group: Molecular Biology
Category: cDNA synthesis
Subcategory: Kits
Application: Other Application
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41105903
Storage instructions: -20-¦C
Scientific information: 
Scientific info: ABScript II cDNA First Strand Synthesis Kit features two optimized mixes, ABScript II Enzyme Mix and ABScript II Reaction Mix. The enzyme mix combines ABScript II Reverse Transcriptase and RNase Inhibitor, while the reaction mix contains an optimized buffer. ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 50 degree C, providing higher specificity and higher yield of cDNA.; The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.
Additional information: 
Synonyms: MBS9141671