HighYield T7 ARCA mRNA Synthesis Kit
ORB611243
Product group Chemicals
Overview
- SupplierBiorbyt
- Product NameHighYield T7 ARCA mRNA Synthesis Kit
- Delivery Days Customer16
- CertificationResearch Use Only
- Scientific DescriptionHighYield T7 ARCA mRNA Synthesis Kit is designed to produce large amounts of ARCA-capped (m)RNA via in vitro transcription with T7 RNA polymerase. The resulting 5-capped (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. Anti-reverse cap analog (ARCA, m7, 3-OGpppG)-capped (m)RNA possesses a significantly higher translation efficiency compared to traditional m7GpppG-capped (m)RNA. This is due to 3-O-methylation of the m7guanine moiety that allows ARCA incorporation in the cor-rect (anti-reverse) orientation only resulting in a 100% translatable capped (m)RNA population. The kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) a 20 microl (6 mM ARCA, 1.5 mM GTP, 7.5 mM CTP, 7.5 mM UTP, 7.5 mM ATP). An individual optimization of ARCA concentra-tion or incorporation of modified nucleotides (e.g. Pseudo-UTP or N4-Acetyl-CTP) up to 100% substitution can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 30-50 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation)).
- Storage Instruction-20°C
- UNSPSC12352200