HighYield T7 ARCA mRNA Synthesis Kit

Biorbyt

HighYield T7 ARCA mRNA Synthesis Kit

16

Research Use Only

HighYield T7 ARCA mRNA Synthesis Kit is designed to produce large amounts of ARCA-capped (m)RNA via in vitro transcription with T7 RNA polymerase. The resulting 5-capped (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. Anti-reverse cap analog (ARCA, m7, 3-OGpppG)-capped (m)RNA possesses a significantly higher translation efficiency compared to traditional m7GpppG-capped (m)RNA. This is due to 3-O-methylation of the m7guanine moiety that allows ARCA incorporation in the cor-rect (anti-reverse) orientation only resulting in a 100% translatable capped (m)RNA population. The kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) a 20 microl (6 mM ARCA, 1.5 mM GTP, 7.5 mM CTP, 7.5 mM UTP, 7.5 mM ATP). An individual optimization of ARCA concentra-tion or incorporation of modified nucleotides (e.g. Pseudo-UTP or N4-Acetyl-CTP) up to 100% substitution can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 30-50 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation)).

-20°C

12352200