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HOT FIREPol(r) DNA Polymerase

HOT FIREPol(r) DNA Polymerase

Research Use Only
01-02
Solis BioDyne
Product group Molecular Biology
Price on request
Packing Size
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Overview

  • Supplier
    Solis BioDyne
  • Product Name
    HOT FIREPol(r) DNA Polymerase
  • Delivery Days Customer
    9
  • Certification
    Research Use Only
  • Scientific Description
    HOT FIREPol® is a chemically modified FIREPol® DNA Polymerase. HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5).
  • UNSPSC
    41116133