Protein A Resin

Product specifications for - Protein A Resin

Overview:
Product group:	Molecular Biology
Category:	Protein / lipid extraction / purification
Subcategory:	Columns / resins

Properties:
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	41106510

Scientific information:
Scientific info:	DescriptionGenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume5 ml settled resin (10 ml 50% slurry)Storage:Store at 4°C, do NOT freeze. Key FeaturesHigh IgG-binding capacityResin is reusable up to 10 times with no significant loss of binding capacity.Characteristics:Ligand Recombinant Streptococcal protein A expressed in E. coliNumber of IgG binding sites per ligand 5MW of ligand Approximately 34 kDaPI of ligand 5.17Degree of substitution Approximately 2 mg protein A/ml settled resinStatic binding capacity >20 mg human IgG/ml settled resinMatrix sphericalagarose, 4% Average particle size90 um (45-165 um)Storage solution1X PBS containing 20% ethanol Storage & Stability 18 months when stored unopened at 2-8°C

Scientific information:

Scientific info:

DescriptionGenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume5 ml settled resin (10 ml 50% slurry)Storage:Store at 4°C, do NOT freeze. Key FeaturesHigh IgG-binding capacityResin is reusable up to 10 times with no significant loss of binding capacity.Characteristics:Ligand Recombinant Streptococcal protein A expressed in E. coliNumber of IgG binding sites per ligand 5MW of ligand Approximately 34 kDaPI of ligand 5.17Degree of substitution Approximately 2 mg protein A/ml settled resinStatic binding capacity >20 mg human IgG/ml settled resinMatrix sphericalagarose, 4% Average particle size90 um (45-165 um)Storage solution1X PBS containing 20% ethanol Storage & Stability 18 months when stored unopened at 2-8°C

Additional information:
Synonyms:	L00210; GenScript

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Product specifications for - Protein A Resin

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Protein A Resin

Product specifications for - Protein A Resin