Mouse anti actin alpha-smooth muscle

Nordic-MUbio

Mouse anti actin alpha-smooth muscle

7

alpha-SM1 reacts exclusively with alpha-smooth muscle actin which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope that is recognized by alpha-SM1 is Ac-EEED.

alpha-SM1 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting, immuno-electron microscopy and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:250 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Electron Microscopy, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Electron microscopy;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

1A4

ACTA2

actin alpha 2, smooth muscle

actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; alpha-cardiac actin; cell growth-inhibiting gene 46 protein

Mouse

IgG2a

Actin, aortic smooth muscle

alpha-SM1 (clone 1A4) is a Mouse monoclonal IgG2a antibody derived by fusion of Sp2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the first 10 amino acids of alpha-smooth muscle actin with an ...

Avian, Chicken, Human, Mammals, Monkey, Porcine, Rat, Sheep, Xenopus

Chicken;Caprine;Monkey;Quail;Rat;Sheep;Swine;Xenopus;Human

12352203