Mouse anti CD14

Nordic-MUbio

Mouse anti CD14

7

The CD14 mAb (clone MEM18) recognizes surface CD14 on human monocytes and macrophages as well as on neutrophils. The sensitivity of MEM18 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to

Indirect Immunofluorescence (Staining Procedure) - Mix 20 microl Nordic-MUbio purified antibody with 50 microl whole blood or MNC suspension - Incubate for 15 minutes at 2-8°C - Wash cells with phosphate buffered saline (PBS) - Add to cell pellet 20 microl of affinity purified, fluorochrome labeled F(ab)2 anti mouse Ig antibodies - Incubate for 15 minutes at 2-8°C - Wash cells with phosphate buffered saline (P and analyse by flow cytometry.

Flow Cytometry, ImmunoFluorescence

Flow Cytometry;Immunofluoresence

Primary antibodies

Research Use Only

Monoclonal

MEM18

CD14

CD14 molecule

monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

Mouse

IgG1

Monocyte differentiation antigen CD14

Human

Human

2°C to 8°C

12352203