Mouse anti Cytokeratin 10+13 / Keratin K10+K13

Nordic-MUbio

Mouse anti Cytokeratin 10+13 / Keratin K10+K13

7

DE-K13 reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with Cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in Keratinizing stRatified epithelia and in differentiated areas of

DE-K13 is useful for immunoblotting, immunocytochemistry, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues (see also above). For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room temperature is required. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

DE-K13

KRT13

keratin 13

CK13; CK-13; cytokeratin 13; K13; keratin 13, type I; keratin, type I cytoskeletal 13; WSN2

Mouse

IgG2a

Keratin, type I cytoskeletal 13

DE-K13 is a Mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal preparation extracted from Human ectocervical epithelium.

Canine, Feline, Human, Zebra Fish

Feline;Human;Zebrafish;Canine

12352203