Mouse anti Cytokeratin 8 / Keratin K8
MUB0318P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rabbit, Rat
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Cytokeratin 8 / Keratin K8
- Delivery Days Customer7
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierFlow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- Clone IDM20
- HostMouse
- IsotypeIgG1
- SourceM20 is a Mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a Mouse immunized with Keratin isolated from the Human breast carcinoma cell line MCF-7.
- ReactivityHuman, Rabbit, Rat
- Reactivity SupplierHuman;Rabbit;Rat
- Storage InstructionThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight ...
- UNSPSC12352203
References
- 1. Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues. Lab Invest 57, 359-69. 2. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 171, 331-45. 3. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of Cytokeratin polypeptides in epithelia of the adult Human urinary tract. Histochemistry 91, 151-9. 4. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136, 657-68. 5. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136, 641-55. 6. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of Cytokeratin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136, 329-43. 7. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). Keratin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis. Cancer Res 50, 5143-52. 8. Wetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related Keratins in Human breast cancer. Am J Pathol 138, 751-63. 9. Ku, N. O., Gish, R., Wright, T. L., and Omary, M. B. (2001). Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med 344, 1580-7. 10. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of Human Keratin 8 following heterotypic association with Keratin 18 and its impliCation for filament stability. Biochemistry 43, 1283-95.