Mouse anti Desmin

Nordic-MUbio

Mouse anti Desmin

7

The antibody D33 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma in human, hamster, rat and chicken. The antibody does not cross react with vimentin or any of

The D33 antibody is suitable for the detection of desmin by Western blotting and detects a band of 53kDa in muscle tissue extracts. The antibody is also suitable for the detection of desmin by immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. The antibody works on paraffin-embedded material without pretreament with proteolytic enzymes, but the section should be pre-treated using heat mediated antigen retrieval with Tris-buffer containing 1 mM EDTA, pH 9,0 for 20 mins. Optimal antibody dilutions for the different applications should be determined by titration.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Immunohistochemistry (frozen);Immunohistochemistry (paraffin)

Primary antibodies

Research Use Only

Monoclonal

D33

DES

desmin

cardiomyopathy, dilated 1F (autosomal dominant); CDCD3; CSM1; CSM2; desmin; epididymis secretory sperm binding protein; intermediate filament protein; LGMD1D; LGMD1E; LGMD2R

Mouse

IgG1

Desmin

D33 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified desmin from a leiomyosarcoma.

Chicken, Hamster, Human, Rat

Human;Hamster;Rat;Chicken

12352203