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Mouse anti double-stranded RNA (J2, J5 and K1) Comparison Set

Research Use Only
10050100
Nordic-MUbio
ApplicationsOther Application, Dot Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
€ 627,00
3 x 100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti double-stranded RNA (J2, J5 and K1) Comparison Set
  • Delivery Days Customer
    7
  • Applications
    Other Application, Dot Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA;Dot blot;Immuno-affinity-chromatography;Immunocytochemistry;Immunohistochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clone ID
    J2, J5 and K1
  • Concentration
    Concentration after reconstitution: 1.00 mg/ml as determined by A280 nm (A280 nm = 1.47 corresponds to 1 mg/ml antibody).
  • Estimated Purity
    Gel electrophoretically pure IgG antibody.
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Anti-dsRNA mAb Comparison Set
  • Source
    Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate t
  • Storage Instruction
    After reconstitution antibodies should be aliquoted and stored at -20 °C or -70°C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for 10 years at -20 °C or -70°C.
  • UNSPSC
    12352203

References

  • 1) F. Weber, V. Wagner, S. B. Rasmussen, R. Hartmann, S. R. Paludan. Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. J Virol (2006), 80(10):5059-64. doi: 10.1128/JVI.80.10.5059-5064.2006. 2) S. Welsch, S. Miller, I. Romero-Brey, A. Merz, C. K. E. Bleck, P. Walther, S. D. Fuller, C. Antony, J. Krijnse-Locker, R. Bartenschlager. Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites. Cell Host & Microbe (2009) 5(4); 365-375. doi.org/10.1016/j.chom.2009.03.007. 3) K. Knoops , M. Bárcena, R. W. Limpens, A. J. Koster, A. M. Mommaas, E. J. Snijder. Ultrastructural characterization of arterivirus replication structures: reshaping the endoplasmic reticulum to accommodate viral RNA synthesis. J Virol. (2012) 86(5); 2474-2487. doi:10.1128/JVI.06677-11. 4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015. 5) K. Karikó, H. Muramatsu, J. Ludwig, D. Weissman, Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA, Nucleic Acids Research (2011) 39(21); e142, https://doi.org/10.1093/nar/gkr695. 6) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000. 7) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272. 8) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford. 9) Levicán-Asenjo J, Soto-Rifo R, Aguayo F, Gaggero A, Leon O. Salmon cells SHK-1 internalize infectious pancreatic necrosis virus by macropinocytosis. J Fish Dis. 2019 Jul;42(7):1035-1046. doi: 10.1111/jfd.13009. Recent publication: Tirosh Shapira, I. Abrrey Monreal, Sébastien P Dion, Mason Jager, Antoine Désilets, Andrea D Olmstead, Thierry Vandal, David W Buchholz, Brian Imbiakha, Guang Gao, Aaleigha Chin, William D Rees, Theodore Steiner, Ivan Robert Nabi, Eric Marsault, Julie Sahler, Avery August, Gerlinde Van de Walle, Gary R Whittaker, Pierre-Luc Boudreault, Hector C Aguilar, Richard Leduc, François Jean. A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice. bioRxiv 2021.05.03.442520; doi: https://doi.org/10.1101/2021.05.03.442520 https://biorxiv.org/cgi/content/short/2021.05.03.442520v1