Mouse anti E-Cadherin / Cadherin-1

Nordic-MUbio

Mouse anti E-Cadherin / Cadherin-1

7

5H9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.

5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues we recommend fixation with 1% formaldehyde in PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 for 10 minutes at room temperature, followed by a permeabilization step with 0.2% Triton X-100 in the same buffer for 5 minutes . For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 9 in a microwave. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting;Immunohistochemistry (paraffin)

Primary antibodies

Research Use Only

Monoclonal

5H9

CDH1

cadherin 1

Arc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin

Mouse

IgG1

Cadherin-1

5H9 is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of ...

Human

Human

12352203