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Mouse anti Human CD3

Research Use Only
0031
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD3E
Price on request
100 ug
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ISO certified
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD3
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2o to 8o C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use as above, however use only 5 microl per test rather than 10 microl. Excites at 488nm and emits at 670nm. Store protected from light.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M2AB
  • Conjugate
    Unconjugated
  • Gene ID916
  • Target name
    CD3E
  • Target description
    CD3e molecule
  • Target synonyms
    CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07766
  • Protein Name
    T-cell surface glycoprotein CD3 epsilon chain
  • Scientific Description
    T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD1E antigen
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes.
  • UNSPSC
    12352203

References

  • 1. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259. 2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293. 3. Signal transduction via CD4,CD8 and CD28 in mature and immature thymocytes. Implications for thymic selection. Turka LA, Linsley PS, Paine R 3d, Schieven Gl, Thompson GB, Ledbetter JA, J. Immunol. 1991 Mar :146(5): 1428-36. 4. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9. 5. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21. 6. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23. 7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. 8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. 9. Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486. Product Specific References: 1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.