Mouse anti Human CD3

Nordic-MUbio

Mouse anti Human CD3

7

PBMC: Add10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2o to 8o C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use as above, however use only 5 microl per test rather than 10 microl. Excites at 488nm and emits at 670nm. Store protected from light.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

M2AB

Unconjugated

CD3E

CD3e molecule

CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; IMD18; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Mouse

IgG1

T-cell surface glycoprotein CD3 epsilon chain

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD1E antigen

See expiration date on vial

Human

Human

CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes.

12352203