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ProductsBack/Mouse anti Human CD34, conjugated with FITC

Mouse anti Human CD34, conjugated with FITC

Research Use Only
0342
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD34
Price on request
100 tests
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD34, conjugated with FITC
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CS37
  • Conjugate
    FITC
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD34=Derived from the hybridization of mouse Sp2/O-Ag14 cells with spleen cells of BALB/c mice immunized with human cell line KG-1a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. Antigenic Analysis of Hematopoiesis III. A Hematopoietic Progenitor Cell Antigen Defined by a Monoclonal Antibody raised against KG-1a cells Civin, C.I., Strauss,L.C., Broveal,C., Fackler,M.J., Schwartz,J.F., and Shaper,J.H., J. Immunol. 133,157,1984 2. Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells. Lebkowski JS; Schain LR; Okrongly D; Levinsky R; Harvey MJ; Okarma T Transplantation 1992 May;53(5):1011-9 3. Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells. Saeland S; Duvert V; Caux C; Pandrau D; Favre C; Valle A; Durand I; Charbord P; de Vries J; Banchereau J Exp Hematol 1992 Jan;20(1):24-33 4. CD34-positive cell proportions in peripheral blood correlate with colony-forming capacity. Fritsch G; Emminger W; Buchinger P; Printz D; Gadner H Exp Hematol 1991 Dec;19(11):1079-83 5. CD34 expression by stromal precursors in normal human adult bone marrow. Simmons PJ; Torok-Storb B Blood 1991 Dec 1;78(11):2848-53 6. Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. Srour EF; Brandt JE; Briddell RA; Leemhuis T; van Besien K; Hoffman R Blood Cells 1991;17(2):287-95 7. Surface marker expression in acute myeloid leukaemia at first relapse. Thomas X; Campos L; Archimbaud E; Shi ZH; Treille-Ritouet D; Anglaret B; Fiere D Br J Haematol 1992 May;81(1):40-4 Product Specific References: 1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.