Mouse Anti Human CD4

Nordic-MUbio

Mouse Anti Human CD4

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CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).

Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 microl/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 microl of the purified monoclonal antibody with RaM FITC conjugate and 100 microl blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.

Flow Cytometry, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin)

Primary antibodies

Research Use Only

Monoclonal

Edu-2

CD4

CD4 molecule

CD4 antigen (p55); CD4 receptor; CD4mut; IMD79; OKT4D; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Mouse

IgG2a

T-cell surface glycoprotein CD4

Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.

Human

Human

12352203