Mouse anti Human CD45RA, conjugated with FITC
045R2
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetPTPRC
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human CD45RA, conjugated with FITC
- Delivery Days Customer7
- Application Supplier NoteAdd 10 microl of MAB / 100 microl of 10^6 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 microl of MAB / 100 microl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
- ApplicationsFlow Cytometry
- Applications SupplierFlow Cytometry
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBHKG-2
- ConjugateFITC
- Gene ID5788
- Target namePTPRC
- Target descriptionprotein tyrosine phosphatase receptor type C
- Target synonymsB220; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
- HostMouse
- IsotypeIgG1
- Protein IDP08575
- Protein NameReceptor-type tyrosine-protein phosphatase C
- Scientific DescriptionCD45RA FITC
- Shelf life instructionSee expiration date on vial
- ReactivityHuman
- Reactivity SupplierHuman
- Reactivity Supplier NoteDerived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- 1. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634 2. CD45 isoform expression on human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM 3. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM 4. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS 5. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H 6. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace DL