Mouse anti Human CD5 FITC - CD20 PE

Nordic-MUbio

Mouse anti Human CD5 FITC - CD20 PE

7

PBMC: Add 10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

FITC, RPE

CD5

CD5 molecule

CD5 antigen (p56-62); epididymis secretory sperm binding protein; LEU1; lymphocyte antigen T1/Leu-1; T1; T-cell surface glycoprotein CD5

Mouse

IgG1, IgG2a

T-cell surface glycoprotein CD5

CD5 FITC - CD20 PE

See expiration date on vial

Human

Human

CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells. CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.

2°C to 8°C

12352203