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ProductsBack/Mouse anti Human CD62L, conjugated with PE

Mouse anti Human CD62L, conjugated with PE

Research Use Only
0624
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetSELL
Price on request
100 tests
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD62L, conjugated with PE
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1H3
  • Conjugate
    RPE
  • Gene ID6402
  • Target name
    SELL
  • Target description
    selectin L
  • Target synonyms
    CD62 antigen-like family member L; CD62L; gp90-MEL; LAM1; LECAM1; LEU8; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; L-selectin; LYAM1; lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; TQ1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP14151
  • Protein Name
    L-selectin
  • Scientific Description
    CD62L PE
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD62L=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with perherial blood T lymphocytes.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. J Cell Biol 1992 Jul;118(2):445-56. Moore KL; Stults NL; Diaz S; Smith DF; Cummings RD; Varki A; McEver RP 2. Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway. Mol Biol Cell 1992 Mar;3(3):309-21. Disdier M; Morrissey JH; Fugate RD; Bainton DF; McEver RP 3. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 1991 May 31;65(5):859-73. Lawrence MB; Springer TA 4. Biochemical and immunohistochemical characteristics of CD62 and CD63 monoclonal antibodies. Expression of GMP-140 and LIMP-CD63 (CD63 antigen) in human lymphoid tissues. Virchows Arch B Cell Pathol 1991;61(4):269-77. Metzelaar MJ; Schuurman HJ; Heijnen HF; Sixma JJ; Nieuwenhuis HK 5. CD62/P-selection recognition of myeloid and tumor cell sulfatides. Cell 1991 Oct 4;67(1):35-44. Aruffo A; Kolanus W; Walz G; Fredman P; Seed B 6. Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1. J Biol Chem 1991 Feb 5;266(4):2466-73. Collins T; Williams A; Johnston GI; Kim J; Eddy R; Shows T; Gimbrone MA Jr; Bevilacqua MP