Mouse anti Human CD8 FITC - CD28 PE

Nordic-MUbio

Mouse anti Human CD8 FITC - CD28 PE

7

PBMC: Add 10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20 C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC and PE. Antibodies should be stored at 4-8oC. Mabs should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

FITC, RPE

CD8A

CD8a molecule

CD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Mouse

IgG1

T-cell surface glycoprotein CD8 alpha chain

CD8 FITC - CD28 PE

See expiration date on vial

Human

Human

CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.

2°C to 8°C

12352203