Mouse Anti-Human IgD Secondary Antibody (clone NI 158, HRP)

LifeSpan BioSciences

Mouse Anti-Human IgD Secondary Antibody (clone NI 158, HRP)

23

Human IgD, isotype specific.

The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgD, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500. in ELISA from 1:1000 upwards. in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. Handling The lyophilized product is shipped at ambient temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. DB, ELISA, ICC, IHC, IHC-P The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgD, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500. in ELISA from 1:1000 upwards. in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. Handling The lyophilized product is shipped at ambient temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Dot Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

NI 158

HRP

Lyophilized

Mouse

IgG1

Human

-20°C,2°C to 8°C

12352203