Mouse anti Human Microphthalmia Transcription Factor (MiTF)
X2398M
ApplicationsGel Shift Assay, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMITF
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human Microphthalmia Transcription Factor (MiTF)
- Delivery Days Customer7
- Application Supplier NoteThis antibody cocktail of clone C5 and D5 can be used for gel supershift assays, immunoprecipitation (2 microg/mg of protein lysate), Western blotting (1 microg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor
- ApplicationsGel Shift Assay, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierImmunohistochemistry (frozen & paraffin);Immunoprecipitation;Western Blotting;Gel Supershift
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDC5/D5 Cocktail
- ConjugateUnconjugated
- Gene ID4286
- Target nameMITF
- Target descriptionmelanocyte inducing transcription factor
- Target synonymsbHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A
- HostMouse
- IsotypeIgG1
- Protein IDO75030
- Protein NameMicrophthalmia-associated transcription factor
- Scientific DescriptionMiTF
- Shelf life instructionSee expiration date on vial
- SourceHybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
- ReactivityHuman, Mouse, Rat
- Reactivity SupplierHuman;Mouse;Rat
- UNSPSC12352203
References
- 1. Weilbaecher KN, et. al. Age-resolving osteopetrosis: a rat model implicating microphthalmia and the related transcription factor TFE3. J. Exp.Med. 1998, 187: 775-785 2. Hemesath P, et. al. MAP kinase links the transcription factor microphthalmia to c-Kit signalling in melanocytes. Nature. 1998, 391:298-301