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Mouse anti Human Microphthalmia Transcription Factor (MiTF)

Research Use Only
X2398M
Nordic-MUbio
ApplicationsGel Shift Assay, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMITF
Price on request
100 ug
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Microphthalmia Transcription Factor (MiTF)
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody cocktail of clone C5 and D5 can be used for gel supershift assays, immunoprecipitation (2 microg/mg of protein lysate), Western blotting (1 microg/ml) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor
  • Applications
    Gel Shift Assay, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (frozen & paraffin);Immunoprecipitation;Western Blotting;Gel Supershift
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C5/D5 Cocktail
  • Conjugate
    Unconjugated
  • Gene ID4286
  • Target name
    MITF
  • Target description
    melanocyte inducing transcription factor
  • Target synonyms
    bHLHe32; class E basic helix-loop-helix protein 32; CMM8; COMMAD; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; microphthalmia-associated transcription factor; MITF-A; WS2; WS2A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO75030
  • Protein Name
    Microphthalmia-associated transcription factor
  • Scientific Description
    MiTF
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human;Mouse;Rat
  • UNSPSC
    12352203

References

  • 1. Weilbaecher KN, et. al. Age-resolving osteopetrosis: a rat model implicating microphthalmia and the related transcription factor TFE3. J. Exp.Med. 1998, 187: 775-785 2. Hemesath P, et. al. MAP kinase links the transcription factor microphthalmia to c-Kit signalling in melanocytes. Nature. 1998, 391:298-301