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Mouse anti Human MyoD1

Research Use Only
X1414M
Nordic-MUbio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Porcine, Rat
TargetMYOD1
Price on request
100 ug
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human MyoD1
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 microg/mg of protein lysate), Western blotting (1 microg/ml) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 microg/ml). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA;Western Blotting;Immunoprecipitation;Immunofluorescense;Immunohistochemistry (frozen & paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5.2F
  • Conjugate
    Unconjugated
  • Gene ID4654
  • Target name
    MYOD1
  • Target description
    myogenic differentiation 1
  • Target synonyms
    bHLHc1; class C basic helix-loop-helix protein 1; MYF3; myf-3; myoblast determination protein 1; MYOD; MYODRIF; myogenic factor 3; PUM
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP15172
  • Protein Name
    Myoblast determination protein 1
  • Scientific Description
    Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
  • Reactivity
    Chicken, Human, Mouse, Porcine, Rat
  • Reactivity Supplier
    Human;Mouse;Rat;Chicken;Pig
  • Reactivity Supplier Note
    Recombinant mouse MyoD1 protein.
  • UNSPSC
    12352203

References

  • 1. Thulasi R; et al. Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth and Differentiation, 1996 Apr, 7(4):531-41 2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9. 3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11. 4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701. 5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81. 7. Kolm PJ and Sive HL. Efficient hormone-inducible proeten function in Xenopus laevis. Developmental Biology, 1995, 171:267-272. 8. Attia GM, Atef H, Elmansy RA. Autologus platelet rich plasma enhances satellite cells expression of MyoD and exerts angiogenic and antifibrotic effects in experimental rat model of traumatic skeletal muscle injury. Egyptian Journal of Histology, 2017, 40: 443-458. 9.Miersch C, Stange K and Roentgen M. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation. BMC Cell Biology, 2018, 19:2