Mouse anti Human MyoD1
X1414M
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Porcine, Rat
TargetMYOD1
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human MyoD1
- Delivery Days Customer7
- Application Supplier NoteThis antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 microg/mg of protein lysate), Western blotting (1 microg/ml) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 microg/ml). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA;Western Blotting;Immunoprecipitation;Immunofluorescense;Immunohistochemistry (frozen & paraffin)
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5.2F
- ConjugateUnconjugated
- Gene ID4654
- Target nameMYOD1
- Target descriptionmyogenic differentiation 1
- Target synonymsbHLHc1; class C basic helix-loop-helix protein 1; MYF3; myf-3; myoblast determination protein 1; MYOD; MYODRIF; myogenic factor 3; PUM
- HostMouse
- IsotypeIgG2a
- Protein IDP15172
- Protein NameMyoblast determination protein 1
- Scientific DescriptionMyoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3
- Shelf life instructionSee expiration date on vial
- SourceHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
- ReactivityChicken, Human, Mouse, Porcine, Rat
- Reactivity SupplierHuman;Mouse;Rat;Chicken;Pig
- Reactivity Supplier NoteRecombinant mouse MyoD1 protein.
- UNSPSC12352203
References
- 1. Thulasi R; et al. Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth and Differentiation, 1996 Apr, 7(4):531-41 2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9. 3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11. 4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701. 5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9. 6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81. 7. Kolm PJ and Sive HL. Efficient hormone-inducible proeten function in Xenopus laevis. Developmental Biology, 1995, 171:267-272. 8. Attia GM, Atef H, Elmansy RA. Autologus platelet rich plasma enhances satellite cells expression of MyoD and exerts angiogenic and antifibrotic effects in experimental rat model of traumatic skeletal muscle injury. Egyptian Journal of Histology, 2017, 40: 443-458. 9.Miersch C, Stange K and Roentgen M. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation. BMC Cell Biology, 2018, 19:2