Mouse anti Lactoferrin, conjugated to PE

Nordic-MUbio

Mouse anti Lactoferrin, conjugated to PE

7

The LF mAb (clone 4C5) recognizes Lactoferrin stored within secondary granules of postmitotic granulocyte-committed cells. The sensitivity of 4C5 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilu

Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM® (Cat. NoGAS-002) intracellular Lactoferrin can be easily stained in cell suspensions. - For each sample to be analyzed add 50 microl of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube - Add 100 microl of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100 microl Reagent B (Permeabilization Medium) and 20 microl of the Lactoferrin monoclonal antibody conjugate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze fixed cells within 24 hours. Conventional Staining for Microscopic Evaluations LF-Antibody 4C5 can also be used to demonstrate lactoferrin molecules by conventional immunofluorescence or immunoenzyme staining techniques on cell smears, cytospin preparations or tissue sections. Acetone or paraformaldehyde are suitable fixatives for these purposes.

Flow Cytometry, ImmunoFluorescence

Flow Cytometry;Immunofluoresence;Immunofluoresence

Primary antibodies

Research Use Only

Monoclonal

4C5

RPE

LTF

lactotransferrin

epididymis luminal protein 110; GIG12; growth-inhibiting protein 12; HEL110; HLF2; kaliocin-1; lactoferricin; lactoferroxin; lactotransferrin; LF; neutrophil lactoferrin; talalactoferrin

Mouse

IgG1

Lactotransferrin

Human

Human

2°C to 8°C

12352203