Mouse anti p21/WAF1/ cip1/Cyclin-dependent kinase inhibitor 1

Nordic-MUbio

Mouse anti p21/WAF1/ cip1/Cyclin-dependent kinase inhibitor 1

7

The antibody WA1 is directed against human p21/WAF1, but also recognizes this protein in non-human primates . Reports suggest that this antibody recognizes an epitope within the carboxy-terminal region of p21/WAF1.

The WA1 antibody is suitable for the detection of p21/WAF1 by flow cytometry, immunocytochemistry, ELISA and western blotting. The antibody is also suitable for the detection of p21/WAF1 by immunohistochemistry of frozen and paraffin embedded tissues. Paraffin embedded tissues require pre-treatment prior to staining with the WA1 antibody. Antigen retrieval by heat treatment using sodium citrate buffer is suggested for this purpose. Optimal antibody dilutions for the different applications should be determined by titration.

Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;ELISA

Primary antibodies

Research Use Only

Monoclonal

WA1

Mouse

IgG1

WA1 is a mouse IgG1 monoclonal antibody derived from the fusion of SP2 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human p21 protein.

Human, Primate

Human;non human primates

12352203