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ProductsBack/Mouse anti P53 (TP53 tumor suppressor protein)

Mouse anti P53 (TP53 tumor suppressor protein)

Research Use Only
MUB1501P
Nordic-MUbio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTP53
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti P53 (TP53 tumor suppressor protein)
  • Delivery Days Customer
    7
  • Application Supplier Note
    The DO-1 antibody is suitable for the detection of p53 by Western blotting and detects a band of 53kDa in tumor tissue and cell extracts. The antibody is suitable for immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The antibody can also be applied in ELISA for the quantification of the p53 protein. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range is 1:500 - 1:1000 for flow cytometry, 1:500-1:2000 for immunohistochemistry, and 1:500 - 1:1000 for immunoblotting applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA;Flow Cytometry;Immunoblotting;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
  • Category Supplier
    Primary Antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DO-1
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Source
    DO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

References

  • 1. Vojtĕsek B, Bártek J, Midgley CA, Lane DP. An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J Immunol Methods. 1992;151(1-2):237-44. 2. Vojtĕsek B, Fisher CJ, Barnes DM, Lane DP. Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique. Br J Cancer. 1993;67(6):1254-8. 3. Stephen CW, Helminen P, Lane DP. Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions. J Mol Biol. 1995;248(1):58-78.