Mouse anti P53 (TP53 tumor suppressor protein)

Nordic-MUbio

Mouse anti P53 (TP53 tumor suppressor protein)

7

The DO-1 antibody is suitable for the detection of p53 by Western blotting and detects a band of 53kDa in tumor tissue and cell extracts. The antibody is suitable for immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The antibody can also be applied in ELISA for the quantification of the p53 protein. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range is 1:500 - 1:1000 for flow cytometry, 1:500-1:2000 for immunohistochemistry, and 1:500 - 1:1000 for immunoblotting applications.

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

ELISA;Flow Cytometry;Immunoblotting;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Research Use Only

Monoclonal

DO-1

TP53

tumor protein p53

BCC7, BMFS5, LFS1, P53, TRP53, cellular tumor antigen p53, antigen NY-CO-13, mutant tumor protein 53, phosphoprotein p53, transformation-related protein 53, tumor protein 53, tumor supressor p53

Mouse

IgG2a

Cellular tumor antigen p53

DO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.

Human

12352203