Mouse anti P53 (TP53 tumor suppressor protein)
MUB1501P
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierNordic-MUbio
- Product NameMouse anti P53 (TP53 tumor suppressor protein)
- Delivery Days Customer7
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierELISA;Flow Cytometry;Immunoblotting;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
- Category SupplierPrimary Antibodies
- CertificationResearch Use Only
- Clone IDDO-1
- HostMouse
- IsotypeIgG2a
- SourceDO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage InstructionThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
- UNSPSC12352203
References
- 1. Vojtĕsek B, Bártek J, Midgley CA, Lane DP. An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J Immunol Methods. 1992;151(1-2):237-44. 2. Vojtĕsek B, Fisher CJ, Barnes DM, Lane DP. Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique. Br J Cancer. 1993;67(6):1254-8. 3. Stephen CW, Helminen P, Lane DP. Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions. J Mol Biol. 1995;248(1):58-78.