Mouse anti Tenascin C

Nordic-MUbio

Mouse anti Tenascin C

7

In immunoblotting assays on an extract of a human osteosarcoma cell line (U2OS) T2H5 recognizes a 265 kDa protein. Tenascin C contains an exstensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than

T2H5 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunohistochemistry (frozen & paraffin);Immunocytochemistry;Immunoprecipitation;Western blotting;Immunohistochemistry (frozen & paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

T2H5

TNC

tenascin C

150-225; cytotactin; deafness, autosomal dominant 56; DFNA56; glioma-associated-extracellular matrix antigen; GMEM; GP; GP 150-225; hexabrachion (tenascin); HXB; JI; myotendinous antigen; neuronectin; tenascin; tenascin-C additional domain 1; TN; TN-C

Mouse

IgG1

Tenascin

T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumour specimen.

Human

Human

12352203