Mouse CD3E + CD3D Protein (Recombinant) (Met1-Ala105)

LifeSpan BioSciences

Mouse CD3E + CD3D Protein (Recombinant) (Met1-Ala105)

14

Research Use Only

>90%

The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 (38+38) kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions. A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.

-80°C

12352202