Mouse IgG2a FITC Negative Control

Nordic-MUbio

Mouse IgG2a FITC Negative Control

7

Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Control antibodies

Research Use Only

FITC

Mouse

IgG2a

See expiration date on vial

2°C to 8°C

12352203