Mouse LIF Protein, His Tag (MALS verified)

ACRObiosystems

Mouse LIF Protein, His Tag (MALS verified)

4

Cytokines&Growth Factors

Research Use Only

Unconjugated

90%

Powder

20.8 kDa

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.8 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Mouse LIF, His Tag (LIF-M5227) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).

Mouse

Mouse

C-6×His. HEK293. Ser 24 - Phe 203

-20℃

12352202