Anti-Hu CD49d Purified Low Endotoxin

EXBIO

Mouse Monoclonal to CD49d / Integrin alpha 4

7

The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not plate

Functional application: In vitro T cell costimulation. Flow cytometry: Recommended dilution: 1-4 microg/ml

Functional Assay, Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Research Use Only

9F10

1 mg/ml

IgG1

CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyers patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.

Bovine, Canine, Equine, Feline, Human, Primate, Sheep

2°C to 8°C

12352203