Anti-Hu CD50 Purified Low Endotoxin

EXBIO

Mouse Monoclonal to CD50 / ICAM-3

7

The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocyte

Functional application: The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1). Flow cytometry: Recommended dilution: 1-4 microg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 microg/ml; positive tissue: tonsil.

Functional Assay, Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

MEM-04

1 mg/ml

IgG2b

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

Human

2°C to 8°C

12352203