Anti-Hu CD64 Purified Low Endotoxin

EXBIO

Mouse Monoclonal to CD64 / FcgammaRI

7

The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to forma

Functional application: Blocking of IgG binding to the FcgammaR1. Flow cytometry: Recommended dilution: 1-4 microg/ml. Immunohistochemistry (paraffin sections and frozen sections): There can occur problems with paraformaldehyde fixation.

Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

10.1

1 mg/ml

IgG1

CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.

Human, Primate

2°C to 8°C

12352203