Chromatin Immunoprecipitation (ChIP) using GeneTex Affinity Purified Mre11 (S. cerevisiae) antibody (GTX12159). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR GreenR (Invitrogen), and detected using a Fuji scanning fluorometer.
Chromatin Immunoprecipitation (ChIP) using GeneTex Affinity Purified Mre11 (S. cerevisiae) antibody (GTX12159). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR GreenR (Invitrogen), and detected using a Fuji scanning fluorometer.
Mre11 antibody
GTX12159
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
ReactivityYeast
TargetMRE11
Overview
- SupplierGeneTex
- Product NameMre11 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:10000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.17 mg/ml
- ConjugateUnconjugated
- Gene ID4361
- Target nameMRE11
- Target descriptionMRE11 homolog, double strand break repair nuclease
- Target synonymsATLD; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11-like protein A; MRE11A; MRE11B
- HostRabbit
- IsotypeIgG
- Protein IDP49959
- Protein NameDouble-strand break repair protein MRE11
- Scientific DescriptionMre11 is an 80 kDa protein that acts as a Mn2+ dependent double stranded DNA 3 to 5 exonuclease and a single stranded DNA endonuclease. It is part of a larger complex involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.
- ReactivityYeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- MRE11 function in response to topoisomerase poisons is independent of its function in double-strand break repair in Saccharomyces cerevisiae. Hamilton NK et al., 2010 Oct 28, PLoS OneRead more