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ProductsBack/MRE11 Rabbit Polyclonal Antibody
WB using Anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4?. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.
WB using Anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4?. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.
WB using Anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4?. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.

MRE11 Rabbit Polyclonal Antibody

TA319244
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetMRE11
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MRE11 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4361
  • Target name
    MRE11
  • Target description
    MRE11 double strand break repair nuclease
  • Target synonyms
    ATLD, HNGS1, MRE11A, MRE11B, double-strand break repair protein MRE11, AT-like disease, DNA recombination and repair protein, MRE11 double strand break repair nuclease A, MRE11 homolog 1, MRE11 homolog A, double strand break repair nuclease, MRE11 homolog, double strand break repair nuclease A, MRE11 meiotic recombination 11 homolog A, MRE11 meiotic recombination 11-like protein A, double-strand break repair protein MRE11A, endo/exonuclease Mre11, meiotic recombination 11 homolog 1, meiotic recombination 11 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49959
  • Protein Name
    Double-strand break repair protein MRE11
  • Scientific Description
    Rabbit polyclonal anti-Mre11 antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MRE11 using anti-MRE11 antibody (A00731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRE11 antigen affinity purified polyclonal antibody (Catalog # A00731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRE11 at approximately 81KD. The expected band size for MRE11 is at 81KD.
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