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ProductsBack/MYD88 Antibody (clone OTI2B2)

MYD88 Antibody (clone OTI2B2)

LS-C172372
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMYD88
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MYD88 Antibody (clone OTI2B2)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:100), IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2B2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4615
  • Target name
    MYD88
  • Target description
    MYD88 innate immune signal transduction adaptor
  • Target synonyms
    IMD68; mutant myeloid differentiation primary response 88; MYD88D; myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYD88 using anti-MYD88 antibody (A00025-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # A00025-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 37 kDa. The expected band size for MYD88 is at 37 kDa.
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