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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA993829(MYF5 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA993829(MYF5 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA993829(MYF5 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200)

MYF5 Antibody

CSB-PA993829
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMYF5
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYF5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4617
  • Target name
    MYF5
  • Target description
    myogenic factor 5
  • Target synonyms
    bHLHc2; class C basic helix-loop-helix protein 2; EORVA; myf-5; myogenic factor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13349
  • Protein Name
    Myogenic factor 5
  • Scientific Description
    Myogenic factor 5 is a protein that in humans is encoded by the MYF5 gene. It is a protein with a key role in regulating muscle differentiation or myogenesis. Without Myf5 and MyoD, myogenic cells will fail to progress normally during the determination stage of myogenesis. Myf5 belongs to a family of proteins known as myogenic regulatory factors (MRFs). These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. MRF family members include Myf5, MyoD (Myf3), myogenin, and MRF4 (Myf6).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MYF5 Polyclonal Antibody, Unconjugated (bs-6936R) at 1:200 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of Myf5 using anti-Myf5 antibody (A04040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 cell lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myf5 antigen affinity purified polyclonal antibody (Catalog # A04040-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myf5 at approximately 28KD. The expected band size for Myf5 is at 28KD.
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