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MYT1L Antibody

LS-C753735
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMYT1L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MYT1L Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23040
  • Target name
    MYT1L
  • Target description
    myelin transcription factor 1 like
  • Target synonyms
    MRD39; myelin transcription factor 1-like protein; myT1-L; neural zinc finger transcription factor 1; NZF1; ZC2H2C2; ZC2HC4B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYT1L using anti-MYT1L antibody (A07880-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYT1L antigen affinity purified polyclonal antibody (Catalog # A07880-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYT1L at approximately 133 kDa. The expected band size for MYT1L is at 133 kDa.
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