ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 microg/mL and GTX89079 for detection at a concentration of 5 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 microg/mL and GTX89079 for detection at a concentration of 5 microg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
Nanog ELISA Pair
GTX500017
Overview
- SupplierGeneTex
- Product NameNanog ELISA Pair - Nanog antibody Pair
- Delivery Days Customer9
- Application Supplier NoteWe recommend using the capture antibody at a concentration of 5microg/mL and the detection antibody at a concentration of 5microg/mL. Optimal dilutions should be determined experimentally by the researcher.
- ApplicationsELISA
- CertificationResearch Use Only
- FormulationLiquid
- Gene ID79923
- Target nameNANOG
- Target descriptionNanog homeobox
- Target synonymshomeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
- Scientific DescriptionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5-TAAT[GT][GT]-3 or 5-[CG][GA][CG]C[GC]ATTAN[GC]-3 (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203