Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NANOG Monoclonal Antibody, Unconjugated (bsm-51459M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NANOG Monoclonal Antibody, Unconjugated (bsm-51459M) at 1:200 overnight at 4°C, DAB staining.
NANOG Monoclonal Antibody
BSM-51459M
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNanog
Overview
- SupplierBioss Antibodies
- Product NameNANOG Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000), IHC-P(IHC-P=1:400-800), IHC-F(IHC-F=1:400-800), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDN3F1
- Concentration0.5 ug/ml
- ConjugateUnconjugated
- Gene ID71950
- Target nameNanog
- Target descriptionNanog homeobox
- Target synonyms2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; ecat; ecat4; EN; ENK; ES cell-associated protein 4; homeobox protein NANOG; homeobox transcription factor Nanog
- HostMouse
- Protein IDQ80Z64
- Protein NameHomeobox protein NANOG
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203