Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting. ECL was used for detection.
Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting. ECL was used for detection.
NEDD4 Rabbit Polyclonal Antibody
TA319441
ApplicationsWestern Blot
Product group Antibodies
ReactivityEquine, Human, Primate
TargetNEDD4
Overview
- SupplierOriGene
- Product NameNEDD4 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID4734
- Target nameNEDD4
- Target descriptionNEDD4 E3 ubiquitin protein ligase
- Target synonymscell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD4-1; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; receptor-potentiating factor 1; RPF1
- HostRabbit
- IsotypeIgG
- Protein IDP46934
- Protein NameE3 ubiquitin-protein ligase NEDD4
- Scientific DescriptionRabbit polyclonal anti-NEDD4 antibody
- ReactivityEquine, Human, Primate
- Storage Instruction-20°C
- UNSPSC12352203