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Western Blot analysis of various samples using NEFH Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NEFH Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using NEFH Polyclonal Antibody at dilution of 1:1000.

NEFH Polyclonal Antibody

E-AB-70063
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NEFH Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    NEFH,also named as KIAA0845 and NFH,Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cells cytoskeleton,and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L,M,and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L,65-68kd; NF-M,145-160kd and NF-H,200-220kd. This antibody recognize NEFH only.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nefh using anti-Nefh antibody (A05307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nefh antigen affinity purified polyclonal antibody (Catalog # A05307) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nefh at approximately 117-220KD. The expected band size for Nefh is at 117KD.
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