Neptune Block ELISA Blocking Buffer

Biorbyt

Neptune Block ELISA Blocking Buffer

16

1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 microL DMSO., 4. Dilute FLICA 1:5 by adding 200 microL PBS., 5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 microL to 290 microL of cultured cells)., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm., If working with adherent cells, please see the manual for additional protocols.

ELISA

Research Use Only

This buffer is Non-Mammalian-based in origin and is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background issues. It is particularly useful with mammalian serum samples, reducing sample-blocker interactions.

2°C to 8°C

41116158