Neptune Sample Diluent

Biorbyt

Neptune Sample Diluent

16

1. AO may be used neat or diluted in diH2O, PBS, or media. ICTs AO is supplied at 0.5 mL liquid at 1 mM (266 microg/mL). 2. Add AO to the cell sample media at 0.5-5 microM, equal to a final dilution of 1:2,000 - 1:200 in the cells (.05-.5% v/v). For example, if using AO at 1.0 microM in the final cell suspension, it must be diluted 1:1,000. First dilute it 1:100 in PBS or diH2O; e.g., put 10 microL AO into 990 microL PBS or diH2O. Pipette the diluted AO into the cell suspension at approximately 1:10; e.g., put 50 microL diluted AO into 450 microL cell suspension., 3. Incubate 15-30 minutes at 37°C., 4. Wash cells if reagent is too bright., 5. Analyze with fluorescence. Lysosomes will appear yellowish green by illuminating cells with a blue light (488 nm) excitation filter and a green light (540-550 nm) emission/barrier filter. Alternatively, lysosomes will appear red when using an excitation filter of 550 nm (540-560 nm) and a long pass > 610 nm emission/barrier filter.

Research Use Only

A non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine in an antigen-down format. Also suitable for human or other mammalian plasma samples in antibody-sandwich ELISAs.

2°C to 8°C

41116133