WB using Anti-Nestin antibody shows detection of a band ~220 kDa corresponding to human Nestin (arrowhead). Undifferentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel.The primary antibody diluted to 1:2,000. Peroxidase conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10000.